The Role of Nitric Oxide on Pancreatic Exocrine Function in Cholecystokinin Stimulated Pancreas

Kim, Kwang Hee; Lee, Hong Sik; Kim, Chang Duck; Lee, Sung Joon; Lee, Goo; Jeen, Yoon Tae; Chun, Hoon Jai; Song, Chi Wook; Um, Soon Ho; Lee, Sang Woo; Choi, Jai Hyun; Ryu, Ho Sang; Hyun, Jin Hai

Korean J Pancreas Biliary Tract > Volume 3(2): > Article

Original Article

산화질소가 Cholecystokinin으로 자극된 췌장외분비에 미치는 영향

김광희, 이홍식, 김창덕, 이성준, 이구, 진윤태, 전훈재, 송치욱, 엄순호, 이상우, 최재현, 류호상, 현진해

고려대학교 의과대학 내과학교실, 소화기연구소

The Role of Nitric Oxide on Pancreatic Exocrine Function in Cholecystokinin Stimulated Pancreas

Kwang Hee Kim, Hong Sik Lee, Chang Duck Kim, Sung Joon Lee, Goo Lee, Yoon Tae Jeen, Hoon Jai Chun, Chi Wook Song, Soon Ho Um, Sang Woo Lee, Jai Hyun Choi, Ho Sang Ryu, Jin Hai Hyun

Department of Internal Medicine, Institute of Digestive Disease and Nutrition, Korea University College of Medicine, Seoul, Korea

Corresponding author: Chang Duck Kim,

ABSTRACT

Background/Aims:
Nitric oxide (NO) is a noxious, unstable vasodilator formed by NO synthase (NOS) from L-arginine (L-Arg) in various cells but its role in the control of pancreatic exocrine secretion has not been fully examined. This study was designed to determine the role of endogenous NO in the control of exocrine pancreas on resting and cholecystokinin (CCK)-stimulated state.
Methods:
Thirty-two male Sprague-Dawley rats (250-300 g/weight) were prepared with jugular vein cannulation and pyloric ligation. Pancreatic duct was cannulated with PE-10 tube and bile duct was cannulated with PE-10 tube for diverging of bile juice. For a resting state and CCK stimulated state, each rat recieved an intravenous (lV) infusion of 0.5M Nacl (1.56 ml/hr) or CCK (50-500 mg/kg/hr) for 1 hour, respectively. L-NAME (5 mg/kg) and L-Name (5 mg/kg/hr) plus L-arginine (100 mg/kg) were administered in bolus at 30 minutes after the onset of NaCl or CCK infusion.
Results:
In resting state, the pancreatic secretory volume and protein output were significantly suppressed by L-NAME (20.8±2.7 vs 14.7±2.6 μl/30 min, 110±12 vs 60±8 μg/30 min, respectively). The suppression of pancreatic volume by L-NAME was reversed by IV administration of L-NAME plus L-arginine. In CCK-stimulated state at 50 and 100 ng/kg/hr, the pancreatic volume and protein output were not changed by IV administration of L-NAME, but the pancreatic secretory volume and protein output were significantly decreased by L-NAME in 500 ng/kg/hr of CCK. The pancreatic secretory volume and protein output were also increased by IV administration of L-NAME plus L-arginine in 500 ng/kg/hr of CCK. Conclusion: These results suggest that nitric oxide involve in resting exocrine pancreatic secretion and CCK-stimulated state at 500 ng/kg/hr, but not in 50 and 100 ng/kg/hr of CCK.

Keywords: Nitric oxide, Pancreatic exocrine function, CCK