1Department of Surgery, Dankook University College of Medicine , Chunan, Korea 2Department of Internal Medicine, Dankook University College of Medicine , Chunan, Korea 3Life Sciences Institute Research Center for Gastroenterology, Dankook University College of Medicine , Chunan, Korea
Corresponding author:
Jung Taik Kim,
ABSTRACT
Background/Aims: H. pylori is very sensitive in vitro to bile acid, but recent reports have noticed that the growth of H. pylori was not affected in bile reflux gastritis, and H. pylori DNA was detected in the human bile from diseased bile duct. Under the certain pathological conditions, altered bile component could cause to change inhibitory factors for the growth of H. pylori in vivo. We investigated if H. pylori could grow in human bile. Methods: The growth rate was studied in 10%, 20%, 50% and 90% of human bile from twenty cases with bile duct disease(17 of bile duct stone, 3 of bile duct cancer). And the morphological change of H. pylori was also studied by 3 hour incubation of active rod formed H. pylori in 10%, 20% and 50% bile. Results: H. pylori isolate was grown in 19 samples of 10% bile, in 13 of 20% bile, and in 2 samples of 50% and 90% bile, respectively. And morphological change from rod to obvious coccoid was observed after 3 hours incubation in 20% bile showing inhibitory effect to H. pylori growth. But there was no difference in morphology in 20% bile showing growth of H. pylori. Conclusion:H. pylori is possible in human bile from the diseased bile duct, and thereby this organism could cause bile duct diseases. Further studies will be needed if there can be difference in H. pylori protein between the bile resistant and bile susceptible H. pylori.
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