1Department of Internal Medicine, Institute of Digestive Disease and Nutrition, Korea University College of Medicine Seoul, Korea 2Department of Anatomic Pathology, Korea University College of Medicine Seoul, Korea
Corresponding author:
Chang Duck Kim,
ABSTRACT
Background/Aims: For identify the tissue injury by oxygen free radical (OFR) in experimental pancreatitis, measurement of lipid peroxidation products has been used. However, the antioxidant defense interact to form as integrated system, therfore, it may be inappropriate to rely on measurement of a single component as an indicator of the functioning of the entire system. Recently, Total Antioxidant Status (TAS) is developed for evaluating the overall performance of the antioxidant system. This study was to assess whether TAS is a useful indicator for evaluating the role of OFR as pathogenesis of experimental pancreatitis. Methods: Fourteen male Sprague-Dawley rat (200-250 g) were used. There were two groups : Control (n=7) and cerulein (n=7) group. Each rat received an iv infusion of cerulein (5 μg/kg/hr) or physiologic saline for 4 hours. After pancreatitis was induced, blood samples for biochemical changes inciuding TAS and glutathion peroxidase (GPx) were taken. Pancreas was prepared for histology. Results: Edematous pancreatitis were induced by cerulein in rat. Serum amylase was significantly elevated in the rats receiving cerulein as compared with controls. Serum TAS and GPx were also significantly decreased in acute edematous pancreatitis. Conclusion: These results suggest that TAS may be a useful indicator as evidence of OFR reaction in the pathogenesis of experimental pancreatitis. Further evaluations are needed to establish the usefulness of TAS.
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